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mouse cd4 t cell subset column kit  (R&D Systems)


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    R&D Systems mouse cd4 t cell subset column kit
    Mouse Cd4 T Cell Subset Column Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse cd4 t cell subset column kit/product/R&D Systems
    Average 92 stars, based on 17 article reviews
    mouse cd4 t cell subset column kit - by Bioz Stars, 2026-02
    92/100 stars

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    R&D Systems mouse t cell cd4 subset column kit
    Splenocytes were treated with S/o and Us/o stimulation and cultured for 1 or 5 days. Cells were stained for viability, <t>CD4</t> and CD25. Only CD25 is depicted here to emphasize that Us/o stimulation produces low levels of CD25 as compared to S/o. Cells were gated on live CD4+ cells and gating was set based on FMO controls. Histograms are representative of triplicate results of at least three separate experiments for each stimulation. NA, naïve (untreated).
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    Splenocytes were treated with S/o and Us/o stimulation and cultured for 1 or 5 days. Cells were stained for viability, CD4 and CD25. Only CD25 is depicted here to emphasize that Us/o stimulation produces low levels of CD25 as compared to S/o. Cells were gated on live CD4+ cells and gating was set based on FMO controls. Histograms are representative of triplicate results of at least three separate experiments for each stimulation. NA, naïve (untreated).

    Journal: Cellular immunology

    Article Title: Cannabidiol (CBD) Induces Functional Tregs in Response to Low-Level T Cell Activation

    doi: 10.1016/j.cellimm.2016.11.006

    Figure Lengend Snippet: Splenocytes were treated with S/o and Us/o stimulation and cultured for 1 or 5 days. Cells were stained for viability, CD4 and CD25. Only CD25 is depicted here to emphasize that Us/o stimulation produces low levels of CD25 as compared to S/o. Cells were gated on live CD4+ cells and gating was set based on FMO controls. Histograms are representative of triplicate results of at least three separate experiments for each stimulation. NA, naïve (untreated).

    Article Snippet: In some experiments, CD4 + T cells were enriched from splenocytes by negative selection using a mouse T cell CD4 subset column kit (R&D Systems, Minneapolis, MN) according to the manufacturer's instructions.

    Techniques: Cell Culture, Staining

    Splenocytes were treated with CBD (0.5-10 μM) or VH (0.1% ethanol) for 30 min followed by low-level stimulation. Cells were stained for viability, CD4, CD25 and FOXP3. Cells are gated on live CD4+ cells. Numbers above plots are average percent gated numbers ± SE of triplicate results for the quadrant. Dot plots are representative of triplicate results of at least three separate experiments for each stimulation. (A) S/o stimulation for 1 day; (B) Us/o stimulation for 5 days; (C) s3/28 for 5 days. * p < 0.05 as compared to VH within each stimulation group. NA, naïve (untreated); Stim, stimulated.

    Journal: Cellular immunology

    Article Title: Cannabidiol (CBD) Induces Functional Tregs in Response to Low-Level T Cell Activation

    doi: 10.1016/j.cellimm.2016.11.006

    Figure Lengend Snippet: Splenocytes were treated with CBD (0.5-10 μM) or VH (0.1% ethanol) for 30 min followed by low-level stimulation. Cells were stained for viability, CD4, CD25 and FOXP3. Cells are gated on live CD4+ cells. Numbers above plots are average percent gated numbers ± SE of triplicate results for the quadrant. Dot plots are representative of triplicate results of at least three separate experiments for each stimulation. (A) S/o stimulation for 1 day; (B) Us/o stimulation for 5 days; (C) s3/28 for 5 days. * p < 0.05 as compared to VH within each stimulation group. NA, naïve (untreated); Stim, stimulated.

    Article Snippet: In some experiments, CD4 + T cells were enriched from splenocytes by negative selection using a mouse T cell CD4 subset column kit (R&D Systems, Minneapolis, MN) according to the manufacturer's instructions.

    Techniques: Staining

    CD4-enriched T cells were treated with CBD (10 μM) or VH (0.1% ethanol) for 30 min followed by Us/o or s3/28 stimulation for 5 days. Cells were stained for viability, CD4, CD25 and FOXP3. Cells are gated on live CD4+ cells. Numbers above plots are average percent gated numbers ± SE of triplicate results for the quadrant. Dot plots are representative of triplicate results of at least three separate experiments. * p < 0.05 as compared to VH within each stimulation group. Stim, stimulated.

    Journal: Cellular immunology

    Article Title: Cannabidiol (CBD) Induces Functional Tregs in Response to Low-Level T Cell Activation

    doi: 10.1016/j.cellimm.2016.11.006

    Figure Lengend Snippet: CD4-enriched T cells were treated with CBD (10 μM) or VH (0.1% ethanol) for 30 min followed by Us/o or s3/28 stimulation for 5 days. Cells were stained for viability, CD4, CD25 and FOXP3. Cells are gated on live CD4+ cells. Numbers above plots are average percent gated numbers ± SE of triplicate results for the quadrant. Dot plots are representative of triplicate results of at least three separate experiments. * p < 0.05 as compared to VH within each stimulation group. Stim, stimulated.

    Article Snippet: In some experiments, CD4 + T cells were enriched from splenocytes by negative selection using a mouse T cell CD4 subset column kit (R&D Systems, Minneapolis, MN) according to the manufacturer's instructions.

    Techniques: Staining

    Splenocytes from FOXP3-GFP mice were treated with CBD (10 μM) or VH (0.1% ethanol) for 30 min followed by Us/o stimulation for 5 days. Cells were stained for viability, CD4 and CD25 without fixation. Cells are gated on live CD4+ cells. Numbers above plots are average percent gated numbers ± SE of triplicate results for the quadrant. Dot plots are representative of triplicate results of at least two separate experiments. * p < 0.05 as compared to VH.

    Journal: Cellular immunology

    Article Title: Cannabidiol (CBD) Induces Functional Tregs in Response to Low-Level T Cell Activation

    doi: 10.1016/j.cellimm.2016.11.006

    Figure Lengend Snippet: Splenocytes from FOXP3-GFP mice were treated with CBD (10 μM) or VH (0.1% ethanol) for 30 min followed by Us/o stimulation for 5 days. Cells were stained for viability, CD4 and CD25 without fixation. Cells are gated on live CD4+ cells. Numbers above plots are average percent gated numbers ± SE of triplicate results for the quadrant. Dot plots are representative of triplicate results of at least two separate experiments. * p < 0.05 as compared to VH.

    Article Snippet: In some experiments, CD4 + T cells were enriched from splenocytes by negative selection using a mouse T cell CD4 subset column kit (R&D Systems, Minneapolis, MN) according to the manufacturer's instructions.

    Techniques: Staining

    Splenocytes were treated with CBD (5 or 10 μM) or VH (0.1% ethanol) for 30 min followed by low-level stimulation. (A) Cells were stimulated with S/o or Us/o stimulation for 1, 3 or 5 days. IL-2 was assessed by ELISA. Data are average of triplicate wells ± SD with * p < 0.05 as compared to VH within group; # p < 0.05 as compared to Day 1. (B, C) Cells were stimulated with S/o (B) or Us/o or s3/CD28 (C) for 1 day. Cells were treated with BFA for the last 4 hr of culture then stained for viability, CD4, IL-2 and TGF-β1. Numbers above plots are average percent gated numbers ± SE of triplicate results for the quadrant. Dot plots represent triplicate results of at least three separate experiments. Cells are gated on live CD4+ cells. * p < 0.05 as compared to VH within each stimulation group. Stim, stimulated.

    Journal: Cellular immunology

    Article Title: Cannabidiol (CBD) Induces Functional Tregs in Response to Low-Level T Cell Activation

    doi: 10.1016/j.cellimm.2016.11.006

    Figure Lengend Snippet: Splenocytes were treated with CBD (5 or 10 μM) or VH (0.1% ethanol) for 30 min followed by low-level stimulation. (A) Cells were stimulated with S/o or Us/o stimulation for 1, 3 or 5 days. IL-2 was assessed by ELISA. Data are average of triplicate wells ± SD with * p < 0.05 as compared to VH within group; # p < 0.05 as compared to Day 1. (B, C) Cells were stimulated with S/o (B) or Us/o or s3/CD28 (C) for 1 day. Cells were treated with BFA for the last 4 hr of culture then stained for viability, CD4, IL-2 and TGF-β1. Numbers above plots are average percent gated numbers ± SE of triplicate results for the quadrant. Dot plots represent triplicate results of at least three separate experiments. Cells are gated on live CD4+ cells. * p < 0.05 as compared to VH within each stimulation group. Stim, stimulated.

    Article Snippet: In some experiments, CD4 + T cells were enriched from splenocytes by negative selection using a mouse T cell CD4 subset column kit (R&D Systems, Minneapolis, MN) according to the manufacturer's instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Staining

    Splenocytes were treated with CBD (1-10 μM) or VH (0.1% ethanol) and Us/o stimulation for 5 days. Cells were treated with BFA for the last 4 hr of culture then stained for viability CD4, IL-10 and/or FOXP3. (A) Intracellular IL-10 in live lymphocytes. Data are average of triplicate wells ± SD with * p < 0.05 as compared to VH. (B) Intracellular IL-10 and FOXP3 in CD4+ T cells. Numbers above plots are average percent gated numbers ± SE of triplicate results for the quadrant. Dot plots represent triplicate results of at least three separate experiments.

    Journal: Cellular immunology

    Article Title: Cannabidiol (CBD) Induces Functional Tregs in Response to Low-Level T Cell Activation

    doi: 10.1016/j.cellimm.2016.11.006

    Figure Lengend Snippet: Splenocytes were treated with CBD (1-10 μM) or VH (0.1% ethanol) and Us/o stimulation for 5 days. Cells were treated with BFA for the last 4 hr of culture then stained for viability CD4, IL-10 and/or FOXP3. (A) Intracellular IL-10 in live lymphocytes. Data are average of triplicate wells ± SD with * p < 0.05 as compared to VH. (B) Intracellular IL-10 and FOXP3 in CD4+ T cells. Numbers above plots are average percent gated numbers ± SE of triplicate results for the quadrant. Dot plots represent triplicate results of at least three separate experiments.

    Article Snippet: In some experiments, CD4 + T cells were enriched from splenocytes by negative selection using a mouse T cell CD4 subset column kit (R&D Systems, Minneapolis, MN) according to the manufacturer's instructions.

    Techniques: Staining

    CD4+CD25+ Tregs and CD4+CD25− T cells from naive mouse splenocytes were purified and treated with CBD (10 μM) plus Us/o stimulation for five days. Cells were stained for viability, CD4, CD25 and FOXP3. Numbers above plots are average percent gated numbers ± SE of triplicate results for the quadrant. Cells are gated on live CD4+ cells. Dot plots are representative of triplicate results from two separate experiments. Average conversion to CD4+CD25+FOXP3+ cells from CD4+CD25− or CD4+CD25+ T cells was 82% or 45%, respectively. Conversion from naive splenocytes was 44%. Conversion rates were calculated as (percentage of CD25+FOXP3+ cells produced by CBD – percentage of CD25+FOXP3+ cells produced by VH)/percentage of CD25+FOXP3+ cells produced by CBD) X 100%. * p < 0.05 as compared to VH within each cell population.

    Journal: Cellular immunology

    Article Title: Cannabidiol (CBD) Induces Functional Tregs in Response to Low-Level T Cell Activation

    doi: 10.1016/j.cellimm.2016.11.006

    Figure Lengend Snippet: CD4+CD25+ Tregs and CD4+CD25− T cells from naive mouse splenocytes were purified and treated with CBD (10 μM) plus Us/o stimulation for five days. Cells were stained for viability, CD4, CD25 and FOXP3. Numbers above plots are average percent gated numbers ± SE of triplicate results for the quadrant. Cells are gated on live CD4+ cells. Dot plots are representative of triplicate results from two separate experiments. Average conversion to CD4+CD25+FOXP3+ cells from CD4+CD25− or CD4+CD25+ T cells was 82% or 45%, respectively. Conversion from naive splenocytes was 44%. Conversion rates were calculated as (percentage of CD25+FOXP3+ cells produced by CBD – percentage of CD25+FOXP3+ cells produced by VH)/percentage of CD25+FOXP3+ cells produced by CBD) X 100%. * p < 0.05 as compared to VH within each cell population.

    Article Snippet: In some experiments, CD4 + T cells were enriched from splenocytes by negative selection using a mouse T cell CD4 subset column kit (R&D Systems, Minneapolis, MN) according to the manufacturer's instructions.

    Techniques: Purification, Staining, Produced

    Us/o + CBD-induced CD4+CD25+ and CD4+CD25− T cells were purified and treated with MMC to prevent proliferation. Fresh responder splenocytes were activated with anti-CD3/28 beads (b3/28) and incubated with MMC-treated, Us/o + CBD-induced cells. The effectiveness of MMC was assessed using fresh splenocytes treated with MMC followed by b3/28 stimulation. Co-cultures were incubated for 4 days. 3H-thymidine was added for last 16 hr of culture. Cells were lysed and 3H-thymidine was collected on glass fiber filters and counted using a scintillation counter. Data are average counts per minute (CPM) of quadruplet wells ± SD. Results are representative of quadruplicate cultures from two separate experiments. a, p < 0.05 as compared to b3/28-stimulated SPLC; b, p < 0.05 as compared to MMC-treated CD4+CD25− T cells plus b3/28-stimulated SPLC.

    Journal: Cellular immunology

    Article Title: Cannabidiol (CBD) Induces Functional Tregs in Response to Low-Level T Cell Activation

    doi: 10.1016/j.cellimm.2016.11.006

    Figure Lengend Snippet: Us/o + CBD-induced CD4+CD25+ and CD4+CD25− T cells were purified and treated with MMC to prevent proliferation. Fresh responder splenocytes were activated with anti-CD3/28 beads (b3/28) and incubated with MMC-treated, Us/o + CBD-induced cells. The effectiveness of MMC was assessed using fresh splenocytes treated with MMC followed by b3/28 stimulation. Co-cultures were incubated for 4 days. 3H-thymidine was added for last 16 hr of culture. Cells were lysed and 3H-thymidine was collected on glass fiber filters and counted using a scintillation counter. Data are average counts per minute (CPM) of quadruplet wells ± SD. Results are representative of quadruplicate cultures from two separate experiments. a, p < 0.05 as compared to b3/28-stimulated SPLC; b, p < 0.05 as compared to MMC-treated CD4+CD25− T cells plus b3/28-stimulated SPLC.

    Article Snippet: In some experiments, CD4 + T cells were enriched from splenocytes by negative selection using a mouse T cell CD4 subset column kit (R&D Systems, Minneapolis, MN) according to the manufacturer's instructions.

    Techniques: Purification, Incubation